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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is noticed in several tumors, which results in chromosome instability, and so might contribute to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial part in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two added splice variants had been described but thorough nucleotide sequence info is not really but available.

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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.

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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...

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This guide outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.

Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose members are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add towards the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for click here cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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